Carbon isotope fractionation during microbial dechlorination. Biodegradation of cisdichloroethene as the sole carbon. The odour thresholds for trans 1, 2 dichloroethene in air and water are 68 mgm3 and 0. Biodegradation of cis 1,2dichloroethylene and vinyl. It can exist as either of two geometric isomers, cis1,2dichloroethene or trans1,2dichloroethene, but is often used as a mixture of the two.
Aerobic biodegradation of vinyl chloride and cis1,2dichloroethylene in aquifer sediments article in chemosphere 6011. Cis1,2dichloroethene has been detected in groundwater samples collected at of the 146 npl sites, in surface water samples collected at 14 of the 146 npl sites, and in leachate samples collected. Although it exhibited the ability under feiiireducing conditions, the chlorinated ethenes degradationwas not linked to the feiii reduction. Oct 06, 2004 read biodegradation of cis 1, 2 dichloroethylene and vinyl chloride in anaerobic cultures enriched from landfill leachate sediment under feiiireducing conditions, biodegradation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Biodegradation of cis1,2dichloroethylene and vinyl chloride. This study focuses on biodegradation of 1,2dichloroethane 1,2dca in micro. Major uses 1,2dichloroethene cistrans mixture is used mainly as an intermediate in the synthesis of chlorinated solvents and compounds 5. Tenant notification fact sheet for 1,2dichloroethene 1,2dce. View products detecting gaseous dichloroethene, cis1,2 tvoc 2 fixed pid detector tvoc 2 is a fixed pid detector for continuous volatile organic compound voc monitoring with leading pid sensor technology and ultimate resistance to humidity and contamination. It can exist as either of two geometric isomers, cis 1, 2 dichloroethene or trans 1, 2 dichloroethene, but is often used as a mixture of the two.
At 20c, strain js666 grew on cdce with a minimum doubling time of 73 7 h. Thermophilic anaerobic biodegradation of tetrachloroethene pce was investigated with various inocula from geothermal and nongeothermal areas. Breathing high levels of 1,2dichloroethene can make you feel nauseous, drowsy, and tired. This threeyear project is to study the anaerobic biological conversion of cis1,2 dichloroethene cdce and vinyl chloride vc to ethene. The study is being conducted in three separate phases, the first to better understand the mechanisms involved in cdce and vc biodegradation, the second to evaluate the chemistry of the processes involved, and the third, to study factors affecting. A thorough understanding of halorespiration, also named organohalide respiration, was obtained 1,4,712. Major uses 1, 2 dichloroethene cis trans mixture is used mainly as an intermediate in the synthesis of chlorinated solvents and compounds 5. Chloroethene concentration and ratios of trichloroethene to cis 1,2dichloroethene and cis 1,2dichloroethene to vinyl chloride concentrations at southern plantation sites mw14, p17, and p19 at operable unit 1, naval undersea warfare center, division keyport, washington, 19912004. Cis1,2dichloroethylene is a highly flammable, colorless liquid with a sharp, harsh odor. Biodegradation of cisdichloroethene as the sole carbon source by. Biodegradation of vinyl chloride, cisdichloroethene and 1,2dichloroethane in the alkenealkaneoxidising mycobacterium strain nbb4.
Bmds modeling summary of relative kidney weight in female rats exposed to cis 1, 2 dce by gavage for 90 days. Enhancing natural attenuation through bioaugmentation. Anaerobic degradation of cis1,2dichloroethylene and vinyl. Aerobic biodegradation of vinyl chloride and cis1,2. Anaerobic degradation of cis1,2dichloroethylene and. Its primary uses are as an industrial solvent and as an intermediate to make other chemicals. Sigmaaldrich offers a number of cis 1,2dichloroethene products. Sigmaaldrich offers a number of cis 1,2dichloroethene solution products. Hydrochemical conditions for aerobicanaerobic biodegradation of. However, tetrachloroethene wasdechlorinated to trichloroethene in 50mmbromoethanesulfonateinhibited enrichments and to trichloroethene and cis1,2dichloroethene in 3 mmfluoroacetateinhibited enrichments. Epa, 2005a, there is inadequate information to assess the carcinogenic potential of cis 1, 2 dce. Aerobic biodegradation of cis1,2dichloroethene as sole. The study is being conducted in three separate phases, the first to better understand the mechanisms involved in cdce and vc biodegradation, the second to evaluate the chemistry of the processes involved, and the third, to study factors. Request pdf bioaugmentation for aerobic degradation of cis1,2dichloroethene polaromonas sp.
Bioaugmentation for aerobic degradation of cis1,2dichloroethene. Mycobacteriumchubuense strain nbb4 can grow on both alkanes and alkenes as carbon sources, and was hypothesised to be an effective bioremediation agent for chlorinated aliphatic pollutants. The most frequent uses of 1,2dichloroethene are solvent production and chemical mixtures 2300. Reductive dechlorination of tetrachloroethene to cis1,2.
During the same period of time aerobic metabolic degradation of vc was extensively studied,14,15,16. Cis1,2dichloroethene cdce is a compound of concern at many chloroethenecontaminated sites, since it tends to accumulate during reductive dechlorination of the higher chlorinated ethenes. Cis 1, 2 dichloroethylene is a highly flammable, colorless liquid with a sharp, harsh odor. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis1,2dichloroethene 5a. Resting cells grown on cdce could transform cdce, ethene, vinyl chloride, trans dichloroethene, trichloroethene, and 1,2dichloroethane. The bacterial community structure of anaerobic enrichment cultures that are capable of degrading both cis1,2dichloroethylene cisdce and vinyl chloride vc and isolation of the organism responsible for the degradation were investigated. Epa, 2005a, there is inadequate information to assess the carcinogenic potential of cis1,2dce.
Read biodegradation of cis1,2dichloroethylene and vinyl chloride in anaerobic cultures enriched from landfill leachate sediment under feiiireducing conditions, biodegradation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Continued biodegradation of chloroethene compounds in. In this study, the ability of nbb4 to biodegrade vinyl chloride vc, cisdichloroethene cdce and 1,2dichloroethane dca was investigated under pureculture. An anaerobic, feiiireducing enrichment culture, which originatedfrom a sediment sample collected at a landfill in nanjido, seoul, korea, was capable ofdegrading cis1,2dichloroethylene cisdce and vinylchloride vc.
An aerobic bacterium capable of growth on cis dichloroethene cdce as a sole carbon and energy source was isolated by enrichment culture. Stable carbon isotope fractionation during aerobic cdce biodegradation was observed in groundwater microcosms under varying incubation conditions room. There is also a potential for release of 1,2dichloroethene to water from hazardous waste sites. Biodegradation of vinyl chloride, cis dichloroethene and 1,2. A stall of cis1,2dce and vinyl chloride vc is frequently observed during. In this study, the ability of nbb4 to biodegrade vinyl chloride vc, cis dichloroethene cdce and 1, 2 dichloroethane dca was investigated under pureculture conditions and in microcosms. Substance details report page 2 of 4 synonym notation t statutesregulations sara 110 sara 110 sdwa npdwr sdwa npdwr tsca inv cis1,2dichloroethylene 1,2dichloroethene, ciscisdichloroethylene cis1,2dichloroethylene ethene, 1,2dichloro, 1zvalid valid valid valid. Enhanced in situ biodegradation of trichloroethene1,1,1 trichloroethane in fractured rock groundwater. Microcosm assessment of a dna probe applied to aerobic.
Tetrachloroethene transformation to trichloroethene cis1,2. An anaerobic, feiiireducing enrichment culture, which originatedfrom a sediment sample collected at a landfill in nanjido, seoul, korea, was capable ofdegrading cis 1, 2 dichloroethylene cis dce and vinylchloride vc. Exposure to 1, 2 dichloroethene occurs mainly in workplaces where it is made or used. It is used to produce solvents and in chemical mixtures. Breathing high levels of 1, 2 dichloroethene can make you feel nauseous, drowsy, and tired. Performing organization names and addresses geosyntec consultants,31. Pdf biodegradation of vinyl chloride, cisdichloroethene and 1,2. The overall removal efficiency under mimicked ates and btes conditions were respectively and 8. Bmds modeling summary of decreased absolute thymus weight in female mice exposed to trans1,2dce in. Degradation of chlorinated organic compounds in general.
Exposure to 1,2dichloroethene occurs mainly in workplaces where it is made or used. The 16s ribosomal dna sequence of the isolate strain js666 had 97. Biodegradation mechanisms reductive dechlorination. Bmds modeling summary of decreased antibody directed against srbc in male mice exposed to trans1,2dce in drinking water for 90 days b14 b6. Page 2 of 43 1 preface 2 3 under the authority of the federal advisory committee act faca p. Biodegradation of vinyl chloride, cis dichloroethene and. May 12, 2010 here, we present the development and evaluation of a dna probe for polaromonas sp. Tenant notification fact sheet for 1,2dichloroethene 1,2. You can smell very small amounts of 1, 2dichloroethene in air about 17 parts of 1, 2dichloroethene per million parts of air 17 ppm. Sequential anaerobicaerobic biodegradation of chloroethenesa. The odour thresholds for trans1,2dichloroethene in air and water are 68 mgm3 and 0. Js666 as a bioaugmentation agent for degradation of cis. Aerobic biodegradation of the chloroethenes oxford academic.
Microcosm assessment of a dna probe applied to aerobic degradation of cis1,2dichloroethene by polaromonas sp. Continued biodegradation of chloroethene compounds in ground. Denaturing gradient gel electrophoresis dgge of a pcramplified 16s rrna gene from the cultures showed the. Cis 1, 2 dichloroethylene is one of two forms of 1, 2 dichloroethylene also called 1, 2 dichloroethene. Enhanced in situ biodegradation of trichloroethene1,1,1.
The most frequent uses of 1, 2 dichloroethene are solvent production and chemical mixtures 2300. Only polluted harbor sediment resulted in a stable enrichment culture that converted pce via trichloroethene to cis1,2dichloroethene at the optimum temperature of 60 to 65c. A mixture of 1, 2 dichloroethene isomers has a pleasant odour 3. Anaerobic microbial reductive dechlorination of tetrachloroethene to predominately trans1, 2dichloroethene. Tetrachloroethene transformation to trichloroethene cis1. J hata, k takamizawa, n miyata, k iwahoribiodegradation of cis 1, 2 dichloroethylene and vinyl chloride in anaerobic cultures enriched from landfill leachate sediment under feiiireducing conditions biodegradation, 14 2003, pp. Compared to the natural situation ns at a constant temperature of 10 c, both utes systems with 255 c for warm and cold well performed significantly better in cisdce cis1,2dichloroethene removal. Technical information for trans1,2dichloroethene and cis1,2dichloroethene cas number. Here, we present the development and evaluation of a dna probe for polaromonas sp. Under the guidelines for carcinogen risk assessment u. Stable carbon isotope fractionation in chlorinated ethene. It occurs in two forms known as cis and trans isomers that have similar properties. Explore information about air pollutants, contaminants and studies being done.
Cometabolism of cis1,2dichloroethene by aerobic cultures grown on vinyl chloride as the primary substrate. A mixture of 1,2dichloroethene isomers has a pleasant odour 3. Mechanisms, chemistry and kinetics of the anaerobic. Stable carbon isotope analysis was performed with a gas chromatographcombustionisotoperatiomassspectrometry systemgccirms10,25. Exposure to 1,2dichloroethene may also result from exposure to other chemicals. It is a highly flammable, colorless liquid with a sharp, harsh odor.
Both the cis and trans forms usually as a mixture are used as a solvent for waxes and resins. However, tetrachloroethene wasdechlorinated to trichloroethene in 50mmbromoethanesulfonateinhibited enrichments and to trichloroethene and cis 1, 2 dichloroethene in 3 mmfluoroacetateinhibited enrichments. Cis1,2dichloroethylene is one of two forms of 1,2dichloroethylene also called 1,2dichloroethene. The epa requires that any spills or accidental release of 1,000 pounds or.
Health information summary 1,2dichloroethene 1,2dce is a synthetic chemical with no known natural sources. Biodegradation of cis1,2dichloroethylene and vinyl. This may be a synopsis of the full weightofevidence narrative. You can smell very small amounts of 1,2dichloroethene in air about 17 parts of 1,2dichloroethene per million parts of air 17 ppm. Download citation aerobic biodegradation of vinyl chloride and cis1,2 dichloroethylene in aquifer sediments laboratory batch experiments have been. Biodegradation of cis1,2dichloroethene in simulated.
Extensive use and inadequate disposal of chloroethenes have led to. Cis 1, 2 dichloroethene cdce is a compound of concern at many chloroethenecontaminated sites, since it tends to accumulate during reductive dechlorination of the higher chlorinated ethenes. Cometabolism of cis1,2dichloroethene by aerobic cultures. As there is currently no evidence for aerobic biodegradation of.